脂多糖对母兔下丘脑、卵巢、输卵管中TLR4及其相关细胞因子表达的影响

doi:10.11843/j.issn.0366-6964.2014.06.008

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 910-917.doi: 10.11843/j.issn.0366-6964.2014.06.008

脂多糖对母兔下丘脑、卵巢、输卵管中TLR4及其相关细胞因子表达的影响

何自标^1§，兰瑞霞^1§，陈晨¹，刘淑君¹，石溢¹，陈仕毅²，赖松家²，张明^1*

（1.四川农业大学动物科技学院，雅安 625014； 2.四川农业大学动物遗传育种研究所，成都 611130）

收稿日期:2014-01-14 出版日期:2014-06-23 发布日期:2014-06-23
通讯作者: 张明，教授，硕士生导师， E-mail:zhm3000@163.com
作者简介:何自标（1987-）男，河南新乡人，硕士生，主要从事动物遗传育种方面研究，E-mail: 645312215@qq.com;兰瑞霞（1989-），女，四川郫县人，硕士生，主要从事动物遗传育种方面研究，E-mail: lrx0089@163.com。何自标和兰瑞霞共同为第一作者
基金资助:
国家人力资源部和社会保障部留学人员科技活动项目；国家兔产业技术体系；四川农业大学“双支”项目

Effect of LPS on the Expression of TLR4 and Related Cytokines in Oviduct，Ovary and Hypothalamus of Rabbits

HE Zi-biao^1§，LAN Rui-xia^1§，CHEN Chen¹，LIU Shu-jun¹，SHI Yi¹，CHEN Shi-yi²，LAI Song-jia²，ZHANG Ming^1*

（1.College of Animal Science and Technology，Sichuan Agricultural University，Ya’an 625014， China;2.Institute of Animal Breeding and Genetics，Sichuan Agricultural University，Chengdu 611130， China)

Received:2014-01-14 Online:2014-06-23 Published:2014-06-23

摘要/Abstract

摘要：

旨在研究革兰阴性菌感染对母兔下丘脑、卵巢和输卵管中TLR4以及其下游炎症细胞因子IL-1β、IL-6表达的影响。选取36只健康新西兰白兔，4只兔不作处理作为0 h组；16只兔作为LPS组，按0.5 mg•kg^-1体重耳缘静脉注射脂多糖（LPS），对照组的16只兔注射等量的生理盐水作为对照组，在注射后0.0、1.5、3.0、6.0和12.0 h(n=4)后收集下丘脑、卵巢和输卵管，采用实时荧光定量PCR分析其TLR4、IL-1β和IL-6的mRNA表达水平，以及采用ELISA测定IL-1β和IL-6在组织中的浓度。结果显示：下丘脑、卵巢和输卵管中均有TLR4的表达，但LPS诱导后，TLR4转录水平仅在卵巢中显著增加（P<0.05），而IL-1β和IL-6的转录水平在下丘脑、卵巢和输卵管中均显著增加（P<0.05），但IL-1β和IL-6对LPS免疫应激在不同器官存在差异：下丘脑对LPS引起的免疫应激反应快，持续时间长；卵巢和输卵管的免疫应激反应慢，持续时间短。结果表明：LPS免疫应激可以上调TLR4、IL-1β和IL-6在下丘脑中以及IL-1β和IL-6在卵巢、输卵管中的表达量。

Abstract:

The aim of this study was to reveal the effect of LPS on expression level of TLR4，and downstream inflammatory cytokines，IL-1β and IL-6 in hypothalamus，oviduct and ovary.36 healthy New Zealand female rabbits，4 animals were non-treatment as 0.0 h，16 animals were injected with LPS according to the dosage of 0.5 mg•kg^-1 body weight as LPS group，and other 16 ones were injected with saline (LPS carrier) as control group.The hypothalamus，ovary and oviduct were collected at 0.0，1.5 ，3.0，6.0 and 12.0 h after injection.The expression level of TLR4，IL-1β and IL-6 were analyzed by real-time PCR and the concentration of IL-1β and IL-6 were examined by ELISA.The results showed：TLR4 was expressed in all experimental tissue samples，but LPS induced the significant up-regulation of TLR4 mRNA only in the ovary (P<0.05).And LPS significantly induced the expression level of IL-1β and IL-6 in the hypothalamus，ovary and oviduct (P<0.05)，but the immunosuppressant of LPS had obviously different in three organs.Namely，immunoresponse in the hypothalamus response to LPS was more sensitive and the duration of immunoresponse was longer than in the ovary and oviduct.According to the results we speculate ：LPS immune stress can increase TLR4，IL-1β，IL-6 expression in the hypothalamus and also increase expression of IL-1β，IL-6 in the ovaries，and oviduct.

中图分类号:

何自标，兰瑞霞，陈晨，刘淑君，石溢，陈仕毅，赖松家，张明. 脂多糖对母兔下丘脑、卵巢、输卵管中TLR4及其相关细胞因子表达的影响[J]. 畜牧兽医学报, 2014, 45(6): 910-917.

HE Zi-biao，LAN Rui-xia，CHEN Chen，LIU Shu-jun，SHI Yi，CHEN Shi-yi，LAI Song-jia，ZHANG Ming. Effect of LPS on the Expression of TLR4 and Related Cytokines in Oviduct，Ovary and Hypothalamus of Rabbits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 910-917.

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脂多糖对母兔下丘脑、卵巢、输卵管中TLR4及其相关细胞因子表达的影响

Effect of LPS on the Expression of TLR4 and Related Cytokines in Oviduct，Ovary and Hypothalamus of Rabbits

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